Enabling Xenopus oocytes and embryos to perform RNAi
نویسندگان
چکیده
منابع مشابه
RNA sorting in Xenopus oocytes and embryos.
Cytoplasmic localization of mRNA molecules has emerged as a powerful mechanism for generating spatially restricted gene expression. This process is an important contributor to cell polarity in both somatic cells and oocytes, and can provide the basis for patterning during embryonic development. In vertebrates, this phenomenon is perhaps best documented in the frog, Xenopus laevis, where polarit...
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The endogenous lectin of Xenopus laevis oocytes, unfertilized eggs, and blastula-stage embryos was immunohistochemically localized using a highly specific antiserum. Each tissue was examined with several techniques, including paraformaldehyde or glutaraldehyde fixation, frozen or plastic sections, and immunofluorescence or immunoperoxidase staining. In oocytes and unfertilized eggs, lectin was ...
متن کاملXenopus Oocytes
lnjection of Xenopus oocytes with poly(A)+ mRNA isolated from different plants (maize, cucumber, and squash) results in the appearance of a voltageand time'dependent, potassium-selective, outward current that is similar to the outwardrectifying potassium current recorded in many higher plant cells. Maize shoots were found to be especially enriched in mRNA encoding such activity. A cDNA library ...
متن کاملXenopus oocytes
The induction of amphibian oocyte maturation with progesterone as well as the activation of sea urchin eggs at the time of fertilization result in increased protein synthesis. The increase in both cases involves the recruitment of maternal mRNA onto polysomes. Further, it has been reported that sea urchin eggs, like full-grown Xenopus oocytes, contain no spare translational capacity based on th...
متن کاملThe stability and movement of mRNA in Xenopus oocytes and embryos.
The Xenopus oocyte has a distinguished history as an in vivo host for the deposition by microinjection of a variety of macromolecules including DNA (Colman, 1984), RNA (Soreq, 1985), proteins (Dingwall, Sharnick & Laskey, 1982) and polysaccharides (Paine, Moore & Horowitz, 1975). In these cases, the oocyte has simply either provided an efficient production system for a product encoded by an inj...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2012
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.26.1_supplement.199.2